Agreement between In-Clinics and Virus Neutralization Tests in Detecting Antibodies against Canine Distemper Virus (CDV)
The Vaccination Guideline Group (VGG) of both the American Animal Hospital Association (AAHA) and the World Small Animal Veterinary Association (WSAVA) recommend puppy core vaccinations followed by triennial antibody titer evaluations of adult dogs.
The gold standard test for measuring canine distemper virus antibody titer to determine level of immunity is virus neutralization (VN), but it is too complex to be performed in a standard veterinary clinic and must be out-sourced. Using in-clinics tests would allow for faster results.
The aim of the study performed by Prof. Dall’Ara et al was to assess the level of agreement between the commercial in-clinic titer test kit “VacciCheck” and VN. Using a VN protective threshold value of 1:32, VacciCheck was calculated to have a sensitivity of 95.5% and accuracy of 92.5%. The Cohen’s kappa coefficient between the two method revealed optimal agreement (p=0073).
The article concludes by stating that VacciCheck is as a reliable titer test that can assist the clinicians in determining the best vaccine protocol, and to avoid unnecessary vaccination.
Sara Meazzi 1,* , Joel Filipe 1 , Alessandra Fiore 1 , Santina Di Bella 2 , Francesco Mira 2 and Paola Dall’Ara