How to Buy
Easy to use
PCRun can easily be used in any vet clinic – no special training is required. If purchasing the lateral flow cassette option, then the molecular detection kits are completely self-contained, without requiring the purchase of any additional accessories.
Rapid results
Receive a point of care PCR result within one hour, requiring only 10 minutes of “hands on” time.
Improves pet-patient care
PCRun allows vets to perform a complete PCR test from the comfort of their own clinics – no need to send samples out to a lab for analysis and then wait.
Within an hour from running the test, the results are ready, accurate and easy to interpret, enabling faster care with greater chances of treatment success.
Highly affordable
With PCRun, you do not have to purchase an expensive, time consuming and complicated thermocycler. Testing in-house can be more cost effective than sending samples off to a lab, making PCRun an effective and affordable molecular detection solution.
PCR testing allows for rapid and highly specific diagnosis of infectious diseases, including those caused by bacteria or viruses.
The high sensitivity of PCR permits earlier detection of acute infections even before the onset of disease. Such early detection gives vets a significant lead time in treatment.
The PCRun methodology is easier to use and the test can be performed simply in a clinic or a lab.
PCRun also provides the same reliability expected from PCR testing. The whole PCRun detection process is no longer than 75 minutes.
PCRun Molecular Detection Kit makes Polymerase Chain Reaction testing:
-
- -Flexible, meaning it can be used in small animal veterinary lab and/or clinic.
- -Faster, more accessible & affordable PCR test
- -No need for expensive devices and multiple steps
Diagnosis in 3 steps:
- DNA Extraction – blood, bone marrow, urine feces, swab
- DNA Amplification
- Results Reading
HEPATOZOON SPECIES
LEUKEMIA VIRUS DNA
MYCOPLASMA
ANAPLASMA
BABESIA CANIS
BABESIA GIBSONI
CANINE BABESIA Species (B. canis, B. vogeli, B. rossi, B. gibsoni, B. negvi)
DISTEMPER RNA
EHRLICHIA
HEPATOZOON SPECIES
PARVOVIRUS / PANLEUKOPENIA
LEISHMANIA
PATHOGENIC LEPTOSPIRA
What is PCR?
PCR is a common molecular tool used for identifying the presence or absence of a specific pathogen.
The high sensitivity of PCR permits earlier detection of acute infections which provides veterinarians with a significant advantage in early treatment.
The accuracy of veterinarian diagnostic PCR testing is undeniable. To-date conventional PCR testing requires multiple steps and expensive complicated devices
with a long turnaround time, typically of 2-4 hour. Until today, PCR could be performed only in specialized laboratories
Why is PCRun different from conventional PCR?
Similar to standard PCR, PCRun can be used for determining the presence of certain pathogens in a clinical sample. The difference is that, PCRun is an isothermal molecular amplification method which simplifies the PCR process and allows for PCR
testing to be performed in a short time. PCRun is more affordable and accessible for clinics and small laboratories.
Should I use serology testing or PCR detection to test for an infectious disease?
Serology and PCR have different windows for diagnosis. PCR is most applicable for the acute phase of disease, prior to, and in the early stages of antibody generation. Serology can be useful in diagnosis during the early and late recovery phase. Taking all the factors mentioned above into account, it must be emphasized that PCR and serology tests do not stand alone when making a final diagnosis and must be used in combination with an accurate history, clinical signs and other diagnostic tests such as biochemistry and haematology testing, in order to come to a final diagnosis.
Where can I use PCRun?
The equipment required to perform PCRun has a small footprint and therefore the test can be performed even in a limited space found in a clinic or a lab.
I need an assistant or technician to performance PCRun?
The methodology is easy to perform, and requires minimal training; most individuals find that PCRun can be performed using the short instruction manual which accompanies each kit.
What do I need to perform PCRun?
PCRun can be performed in a clinic or in a lab; there are two formats, each designed for your specific needs.
PCRun for Clinics PCRun for Labs
PCRun Heat Block PCRun Reader
PCRun Extraction kit PCRun Extraction kit
PCRun Molecular Reaction kit PCRun Molecular Reaction kit
*Though you may find the PCRun for clinics may be more suited for your lab and the PCRun for labs may be more suited for your clinic.
Contact a Biogal representative for any further information- info@biogal.com
What kind of results do I get from PCRun?
When using the “PCRun for Clinics” the qualitative results are revealed on a lateral flow device – ideal for vet clinics.
When using “PCRun for Labs” the results appear in real time on the screen of the PCRun Reader and a semi quantitative interpretation can be derived from the values generated
Which Pathogens can be detected by PCRun?
The PCRun Molecular Detection Kit is currently available for -Pathogenic Leptospira, Ehrlichia canis, Leishmania infantum, Babesia canis, Babesia gibsoni, Anaplasma platys,, Feline and Canine Parvo Virus, Mycoplasma haemofelis and haemocanis, Feline Leukemia Virus and endogenous FeLV and Canine Distemper Virus.
I need any other accessories to perform the tests?
Most of the kits do not require any equipment other than the disposables found in the kit. The Distemper and Leishmania kits do require a suitable centrifuge which can reach and maintain 10,000 X g. Accurate laboratory pipettes with barrier tips are also required to perform these tests.
Contact a Biogal representative for any further information: info@biogal.com
What type of samples do I need to collect?
The samples vary according to the target pathogen. Please refer to the instructions manual or contact your sales representative for assistant.
Does PCRun need special storage?
The PCRun kit can be stored at 2-25°C and the reaction performed at room temperature.
What is the shelf life of the products?
Extraction Kits (DNA or RNA) – up to 12 months from the manufacturing date
Molecular reaction kits – up to 15 months
What is the sensitivity and specific of PCRun results?
PCRun provides the same reliable results as any PCR. Specific information can be acquired from the supplier.
How long does the PCRun reaction take until results are available?
PCRun takes 75 minutes (10 minutes of preparation and 60 minutes of incubation).
Can a patient with clinical signs have negative PCRun results?
Both standard PCR and PCRun testing can result in a false negative result. This can occur when:
(i) the target pathogen concentration is below the limit of detection of the test.
(ii) antibiotics have been administered prior to testing.
(iii) an improper sample for the specific disease is used.
(iv) the sample was not maintained under optimal condition prior to extraction
(v) the test was performed incorrectly.
CANINE EHRLICHIA CANIS
A NEW TAQMAN METHOD FOR THE RELIABLE DIAGNOSIS OF EHRLICHIA SPP. IN CANINE WHOLE BLOOD.
Thomson et al. Parasites & Vectors
USE OF SEROLOGY AND PCR FOR THE DETECTION OF EHRLICHIA CANIS
PCRun Ehrlichia Data
Biogal Data on File
Use of Serology and PCR for the Detection of Ehrlichia canis
CANINE ANAPLASMA PLATYS
THE PCRUN ANAPLASMA PLATYS MOLECULAR DETECTION KIT TESTED BY THE VECTOR BORNE DISEASE DIAGNOSTIC LABORATORY LOCATED IN THE SCHOOL OF VETERINARY MEDICINE, NORTH CAROLINA STATE UNIVERSITY
Biogal Data on File
NCSU Anaplasma sensitivity and specificity 160926
CANINE BABESIA CANIS
ANALYSIS OF PCRUN® CANINE BABESIA CANIS MOLECULAR DETECTION KIT COMPARED TO TAQMAN REALTIME PCR.
- Maurice- Biogal Galed Labs Acs.
Biogal Data on File
MOLECULAR DETECTION OF BABESIA CANIS VOGELI IN DOGS IN THE CITY OF SÃO PAULO
Luís – MA, Brazil
- S. Galeno et al, PUBVET v.12, n.6, a108, p.1-4
detecccedilatildeo-molecular-de-babesia
CANINE BABESIA GIBSONI
PRELIMINARY STUDY OF BABESIA GIBSONI AT THE VECTOR BORNE LABORATORY AT NORTH CAROLINA UNIVERSITY
Biogal Data on File
Preliminary Study of Babesia gibsoni
CANINE PATHOGENIC LEPTOSPIRA
COMPARISON OF A NOVEL POINT-OF-CARE DIAGNOSTIC, PCRUN, WITH REAL TIME LEPTOSPIRA PCR FOR DETECTION OF LEPTOSPIRA ANTIGEN IN CANINE SAMPLES.
R.D. Schultz et al, Abstract-Conference of Research Workers in Animal Diseases.
PCRUN CANINE PATHOGENIC LEPTOSPIRA MOLECULAR DETECTION KIT – INCLUSIVITY AND EXCLUSIVITY STUDY
Kiddle et al.
Biogal Data on File
CANINE LEISHMANIA INFANTUM
EVALUATION OF LEISHMANIA INFANTUM PCRUN® DNA DETECTION KIT
Biogal Data on File
Evaluation of Leishmania infantum PCRun DNA Detection kit
CANINE PARVO VIRUS MOLECULAR
COMPARATIVE CLINICAL VERIFICATION OF PCRUN® PARVOVIRUS MOLECULAR DETECTION KIT
Larson DVM, CAVIDS Titer Testing Laboratory, University of Wisconsin, Madison
Wisconsin.
Biogal Data on File
Comparative Clinical Verification of PCRun Parvovirus (Univ.Wisconsin – April 2018)
IS FECAL SAMPLING THE IDEAL SPECIMEN FOR CANINE PARVOVIRUS PCR DETECTION?
COMPARISON OF A NOVEL POINT OF CARE DIAGNOSTIC WITH REAL TIME PCR
Yaaran1, S. Maurice1, WSVA8-0070.
Abstract from WSAVA 2018
COMPARISON OF A NOVEL POINT OF CARE DIAGNOSTIC WITH REAL TIME PCR
Yaaran1, S. Maurice1, WSVA8-0070.
Abstract from WSAVA 2018
CANINE DISTEMPER
COMPARATIVE CLINICAL VERIFICATION OF PCRUN® CANINE DISTEMPER RNA MOLECULAR DETECTION KIT
Larson DVM, CAVIDS Titer Testing Laboratory, University of Wisconsin, Madison
Wisconsin
Biogal Data on File
COMPARATIVE CLINICAL VERIFICATION OF PCRUN® CANINE DISTEMPER RNA MOLECULAR DETECTION KIT
Larson DVM, CAVIDS Titer Testing Laboratory, University of Wisconsin, Madison
Wisconsin
Biogal Data on File
Comparative Clinical Verification of PCRun Distemper RNA (Univ.Winsconsin)
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