In-clinic titer testing
A comparative study
- 5 independent clinics in the Netherlands
- 3 commercial in-clinic titer test kits
- 3 canine core diseases
Abstract
Objetves
To analyze the performance of three in-clinic test kits for the detection of
protective serum antibody titers against:
Method
46 serum samples from vaccinated dogs – were tested for the presence of protective antibodies using three commercial in-clinic test kits:
Study design and method
Prospective comparative study (using fresh plasma samples)
- 5 veterinary clinics (4 in the Netherlands and one in Belgium).
- 7-10 healthy adult dogs with a known vaccination history.
- Tests performed sequentially. Results recorded in results-form + photographic documentation.
- Aliquot of plasma sent frozen for gold standard iter test (SN and HI) AHDC, Cornell University.
Results
Conclusions
and FasTest have excellent agreement between themselves and with the Gold standard laboratory methods.
The RapidStatus kit generated different to those of , FastTest and the reference laboratory gold standard test.
A high rate of false negatives could lead to unnecessary revaccination of dogs.
Why use ?
Added value
Vaccicheck measures CAV Ab while the FasTest does not. Although some veterinarians dismiss the importance of CAV – it is still part of the WSAVA Guidelines recommendations and is still a relevant disease.
Unlike VacciCheck, FasTest results have no diagnostic value after 20 minutes. This is especially important when there is a need for a second review of the results.
Only Vaccicheck has been evaluated and approved by the USDA (USA) and MAFF (Japan).