Clinical Verification of PCRun®
Aim: To determine the sensitivity and specificity of Biogal’s PCRun® Canine Distemper RNA Molecular Detection Kit as compared to the commercial Reverse Transcription (RT) Distemper Kit designed by Primerdesign ™ Ltd (Cambridge UK).
Background: Canine distemper is a highly contagious lethal viral disease occurring in both domestic and wild canids worldwide. The virus is transmitted by aerosols and direct contact, resulting in fever, serous nasal discharge and cough, as well as respiratory, neurological and gastrointestinal signs complicated by secondary bacterial infections. Molecular tests such as the Polymerase Chain Reaction (PCR) are the most accurate method to diagnose the disease1. Primerdesign Real Time PCR and Biogals’s isothermal
PCRun Distemper RNA Detection Kit are both PCR platforms designed to detect the nucleocapsid (N) gene in clinically derived samples from patients demonstrating acute signs of the Distemper virus.