VacciCheck Technical FAQ´S by Biogal 15337 VacciCheck Technical FAQ´S We answer frequently asked questions about VacciCheck How many tests come in a kit? Each VacciCheck kit contains12 individual tests that can be run one at a time or batch tested up to 12 at a time. A VacciCheck lab pack is available as well, which contains 120 tests. Do I need any additional accessories or equipment to run a test? VacciCheck is a ready to use in-clinic titer test so you don’t need any accessories to run the test in your clinic/lab. Biogal offers three complementary devices for an easier test run: Our Platewarmer, RoboComb and CombCam. For more information, visit: ROBOCOMB COMBCAM Does the kit need to be kept in a refrigerator? VacciCheck should be kept refrigerated at 2-8 °C/26-46 °F when not in use and should be brought to room temperature prior to being used. Refer to the kit’s instructions for the appropriate acclimation time. Is it possible to reduce the time of the test performance? The test warm-up time before use can be reduced by using Biogal’s Platewarmer, for an automation development of the test RoboComb can be used and reading of results can be done by using, Biogal’s CombCam. For more information visit: www.biogal.com How much blood or serum do I need to run the test? The test requires a very small amount of blood: just 5μl of serum/plasma or 10 μl of whole blood. Is a cold sample acceptable for VacciCheck? Before testing, bring the sample to room temperature. Small vials usually require 10 minutes in room temperature before they are ready for use. How long can the sample be kept before performing the test? It is recommended you use fresh samples. Store whole blood at 2-8 °C/26-46 °F if the test is to be run within 1 day of collection. Do not freeze whole blood samples. Store serum and plasma samples at 2-8 °C/26-46 °F if the test is to be run within 3 days of collection. If the test is delayed more than 3 days, freeze samples to -20°C or colder. Bring samples to room temperature and mix well before testing. Is it possible to run a whole batch simultaneously on VacciCheck? Absolutely! some clinics will collect samples throughout the week and keep them refrigerated to run a batch test simultaneously. Each test is self-contained, meaning, if you run 4 tests at a time, the other 8 will still be perfectly fine as long as they remain sealed. What is VacciCheck shelflife? VacciCheck has a shelf life of 15 months from the day of production. Once a kit has passed the expiration date, it can no longer be guaranteed for accuracy and should be properly disposed. Do I have to move the comb in each well? VacciCheck is anELISA-based test that requires the developing comb to be moved throughout the 6 rows and into each well, alternating between two and five minutes (please refer to the Instruction manual for further guidance). Remember: Mixing by moving the comb up and down must be done in all wells. The optimal mixing goes as follows: During each incubation step, perform 2-3 mixing actions. Each mixing action includes moving the comb up and down 3-4 times. Reduce the excess liquid on a paper towel before you move to the next row. Any deviation in the operation of the test will affect the development of the test and the ability to interpret the results correctly. result correctly. Do the results fade over time? No. If the comb is stored in the dark, once the results have been developed, they won’t fade and can be kept on record. What happens if a I accidentally puncture a test that I’m not using? If a test has been punctured, refrain from using it since future contamination may result in an inaccurate test result. Other sealed tests may still be used. What are the recommendations for transportation and storage? VacciCheck products need to be transported and stored at 2-8 °C or 26-46 °F. All products were tested in accelerated extreme conditions to verify their resistance to short uncontrolled conditions that may occur during transportation. During reasonable short-term transport (usually 24-72 hours) the kits can resist exceeding temperatures above the marked range and their quality, in general, will not be affected. What is the reason of a background in the comb? In some cases, a dark background in the comb might appear, which can be related to some of the following cases: Suboptimal mixingHigh immunoglobulin contentSpecimens Abnormality For any inquiries/complaints, please fill the following link and submit your question. We will be happy to assist you. Download Technical FAQS Notice: JavaScript is required for this content. About Biogal