PCRun® FAQ

What is PCR?

PCR is a common molecular tool used for identifying the presence or absence of a specific pathogen.

The high sensitivity of PCR permits earlier detection of acute infections which provides veterinarians with a significant advantage in early treatment.

The accuracy of veterinarian diagnostic PCR testing is undeniable.  To-date conventional PCR testing requires multiple steps and expensive complicated devices

with a long turnaround time, typically of 2-4 hour. Until today, PCR could be performed only in specialized laboratories

Why is PCRun different from conventional PCR?

Similar to standard PCR, PCRun can be used for determining the presence of certain pathogens in a clinical sample. The difference is that, PCRun is an isothermal molecular amplification method which simplifies the PCR process and allows for PCR

testing to be performed in a short time. PCRun is more affordable and accessible for clinics and small laboratories.

Should I use serology testing or PCR detection to test for an infectious disease?

Serology and PCR have different windows for diagnosis. PCR is most applicable for the acute phase of disease, prior to, and in the early stages of antibody generation.  Serology can be useful in diagnosis during the early and late recovery phase. Taking all the factors mentioned above into account, it must be emphasized that PCR and serology tests do not stand alone when making a final diagnosis and must be used in combination with an accurate history, clinical signs and other diagnostic tests such as biochemistry and haematology testing, in order to come to a final diagnosis.

Where can I use PCRun?

The equipment required to perform PCRun has a small footprint and therefore the test can be performed even in a limited space found in a clinic or a lab.

Do I need an assistant or technician to performance PCRun?

The methodology is easy to perform, and requires minimal training; most individuals find that PCRun can be performed using the short instruction manual which accompanies each kit.

What do I need to perform PCRun?

PCRun can be performed in a clinic or in a lab; there are two formats, each designed for your specific needs.

PCRun for ClinicsPCRun for Labs
PCRun Heat BlockPCRun Reader
PCRun Extraction kitPCRun Extraction kit
PCRun Molecular Reaction kitPCRun Molecular Reaction kit

*Though you may find the PCRun for clinics may be more suited for your lab and the PCRun for labs may be more suited for your clinic.

Contact a Biogal representative for any further information- info@biogal.com

What kind of results do I get from PCRun?

When using the “PCRun for Clinics” the qualitative results are revealed on a lateral flow device – ideal for vet clinics.

When using “PCRun for Labs” the results appear in real time on the screen of the PCRun Reader and a semi quantitative interpretation can be derived from the values generated

Which Pathogens can be detected by PCRun?

The PCRun Molecular Detection Kit is currently available for -Pathogenic Leptospira, Ehrlichia canis, Leishmania infantum, Babesia canis, Babesia gibsoni, Anaplasma platys,, Feline and Canine Parvo Virus, Mycoplasma haemofelis and haemocanis, Feline Leukemia Virus and endogenous FeLV and Canine Distemper Virus.

Do I need any other accessories to perform the tests?

Most of the kits do not require any equipment other than the disposables found in the kit. The Distemper and Leishmania kits do require   a suitable centrifuge which can reach and maintain 10,000 X g. Accurate laboratory pipettes with barrier tips are also required to perform these tests.

Contact a Biogal representative for any further information: info@biogal.com

What type of samples do I need to collect?

The samples vary according to the target pathogen.  Please refer to the instructions manual or contact your sales representative for assistant

Does PCRun need special storage?

The PCRun kit can be stored at 2-25°C and the reaction performed at room temperature.

What is the shelf life of the products?

Extraction Kits (DNA or RNA) – up to 12 months from the manufacturing date

Molecular reaction kits – up to 15 months

What is the sensitivity and specific of PCRun results?

PCRun provides the same reliable results as any PCR.  Specific information can be acquired from the supplier.

How long does the PCRun reaction take until results are available?

PCRun takes 75 minutes (10 minutes of preparation and 60 minutes of incubation).

Can a patient with clinical signs have negative PCRun results?

Both standard PCR and PCRun testing can result in a false negative result. This can occur when:

(i)  the target pathogen concentration is below the limit of detection of the test.

(ii)  antibiotics have been administered prior to testing.

(iii) an improper sample for the specific disease is used.

(iv) the sample was not maintained under optimal condition prior to extraction

(v)  the test was performed incorrectly.

PCRun Molecular Detection kit +PCRun Reader